HPLC AND SPECTROFLUORIMETRIC DETERMINATION OF PYRENE IN THE LEBANESE COAST

HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast

Jamil Rima

Laboratory of Physical Chemistry of Environmental Engineering and Biology, Faculty of Science II, P.O. Box 26110217, Lebanese University, Fanar, LEBANON

Abstract

In this article, homogeneous liquid-liquid extraction (HOLLE), combined with HPLC-fluorescence detector (HPLC-FLD and Fluorescence Spectrophotometer, has been developed for the extraction and determination of polycyclic aromatic hydrocarbons (PAHs) in polluted seawater. A liquid-liquid extraction followed by evaporative concentration method was used to determine the concentration of polycyclic aromatic hydrocarbons (PAH) in Lebanese marine water. Hydrocarbons were extracted from 450 ml of water into 75 ml n-hexane and then concentrated into 1 or 2 ml solution by evaporation. Internal standard addition method was used to determine the pyrene. Under the optimum conditions, repeatability was carried out by spiking pyrene at concentration level of 114.5 μg/l. the RSDs varied between 1.1 and 8.5% (n = 3). The LODs, based on S/N of 3, ranged from 0.025 to 0.25 μg/l. The pyrene determined in marine water was generated from the incomplete combustion of fuel oil in the powerplant of Zouk Mikael (Lebanon). Several PAHs were observed but the pyrene was easily indentified and quantified, due to the relative high concentration of this compound.

Keywords: Pyrene, seawater, determination, HPLC, spectrofluorimeter, internal standard addition