METACHROMATIC PROPERTIES OF AGARS AND CARRAGEENAN

Metachromatic properties of agars and carrageenan

Mürşide Sur, Kasım Cemal Güven

Istanbul University, Institute of Marine Sciences and Management, Musküle Sokak 1,34470 Vefa, Istanbul, TURKEY

Abstract

The metachromatic properties of agars obtained from Turkish coast of carrageenan were investigated after eluation on Sepharose 2B. The each 5ml eluate was separated and α-, β- bands were determined with azur A by spectrophotometer. The metachromatic band is shown amongst the volume of the eluates are: for agars; 15-55 ml for Phyllophora nervosa, Gracilaria verrucosa 20-45 mI, Ceramium rubrum 25-55 mI, 15-45ml for Gelidium latifolium and 20-30 mI for Pasteur agar and 25-95 m1 for carrageenan. The results show that the metachromatic properties of agars tested were not similar.

Keywords: Agars, carrageenan, Sepharose 2B, Azure A, metachromasy