Diatoms isolated from the marine mucilage in the Sea of Marmara in 2021

Diatoms isolated from the marine mucilage in the Sea of Marmara in 2021

Dilek Tekdal, Atıf Emre Demet, Elif Eker-Develi, Hüseyin Bekir Yıldız

ORCID IDs: D.T. 0000-0002-4545-9005; A.E.D. 0000-0002-7401-9455; E.E.D. 0000-0003-0174-1903; H.B.Y. 0000-0003-1123-8797

Department of Biotechnology, Faculty of Science and Letters, Mersin University, 33343, Mersin, TÜRKİYE
Department of Energy Systems Engineering, Faculty of Engineering, Necmettin Erbakan University, 42090, Konya, TÜRKİYE
Department of Mathematics and Science Education, Faculty of Education, Mersin University, 33343, Mersin, TÜRKİYE
Department of Mechanical Engineering, Bartın University, Bartın, TÜRKİYE

Abstract

This study presents five new records of diatoms based on light and electron microscopy results from the surface water of the Sea of Marmara coast during a large-scale mucilage event in 2021. Three of these diatoms are small nanoplanktic; Cyclotella cryptica, Nitzschia amabilis, Thalassiosira pseudonana, and the two of them are larger diatoms; Conticribra weissflogii and Entomoneis cf. pusilla. In addition, a species frequently seen in mucilage samples in the Adriatic Sea and the the Sea of Marmara, Cylindrotheca closterium, was also isolated and described. The chloroplast rbcL region, SSU, and 18S rDNA of C. closterium, C. cryptica and N. amabilis were amplified using primers in other previous studies. The rbcL, SSU, and 18S rDNA partial sequences were submitted to the NCBI database. Molecular phylogeny (based on 18S, rbcL, and SSU V4 DNA sequences) confirmed that the analysed species were those identified by SEM images.

Keywords: Centrales, pennales, nanoplankton, SEM, DNA sequences

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