Ecological risk assessment to populations of small benthic Crustaceans

V.G.Tsytsugina, G. G. Polikarpov

The A.O. Kovalevsky Institute of Biology of the Southern Seas, NAS, Sevastopol, 99011, UKRAINE


Small benthic crustaceans are considered in comparison to large ones as the most vulnerable in relation to injurious effects according to proposed by us criteria of “critical” populations (species) identification. Experimental cytogenetic data on enfluence of different mutagene equivalent doses of ionising radiation and chemical mutagens (i.e. doses inducing equal mean number of cells with chromosome aberration in %) on Gammarus olivii and Idothea balticaposterity are analized and compared with results of cytogenetic analysis of natural crustacian populations. For the assessment of specimens reproductive contribution and effective population size (i.e. number of specimens determing genetic structure of next posterities) a number of embryos with spontaneous chromosome mutagenesis (up to 2% of cells with chromosome aberrations) is used as a criterion of postery of full value. It is concluded that cytogenetic analysis of female posterity allows to assess expected reduction of population adaptive possibiliity and ecological risk.

Keywords: Lonising radiation, chemical mutagens, benthic crustaceans, ecological risk

pdf-dl icon


Chubareva L.A. (1985). B-chromosomes in the spermatogenesis of the blackelies. Cytology 27(4): 363-367 (in Russian).

Crow J.F., Kimura M. (1970). An introduction to populations genetic theory. New-York: Harper and Row. 430 p.

Jones D., Domotor S., Higley K., Kocher D., Bilyard G. (2003). Principles and issues in radiological ecological risk assessment. J. Environ. Radioactivity 60: 19-39.

Makkaveeva E.B. (1979). Invertebrates of macrophytes of the Black Sea. Kiev. Publishing House “Naukova dumka”. 228 p. (in Russian).

Patin S.A. (1979). Influence of pollution on biological resources and production of the World Ocean. M. Publishing Hause “Pish. Prom.” 304 p. (in Russian).

Pentreath R.J. (1998). Radiological protection criteria for the natural environment. Radiat. Protection Dosimetry 75 (1-4): 175-179.

Polikarpov G.G., Tsytsugina V.G. (1993). Distribution of chromosome aberrations among hydrobiont cells under the effect of ionizing radiation and chemical mutagen of the environment. Radiobiology 33 (2): 205- 213. (in Russian).

Polikarpov G.G., Tsytsugina V.G. (1996). Study of the after–effect of the Chernobyl NPP accident on hydrobionts populations. Radiation Biology. Radioecology 36 (4): 460-468 (in Russian).

Romashov D.D., Belyaeva V.N. (1966). About constancy of radiation damage chromosomes in fish embryogenesis. Genetica (4): 4-15 (in Russian).

Strand P., Brown J.E., Larson C.M. (2000). Framework for the protection of the environment from ionizing radiation. Radiat. Protection Dosimetry 92 (1 – 3): 169-175.

Tsytsugina V.G. (1979). Chromosome variability in marine organisms under natural conditions (on the problem of biological effect of contaminants). In: Proceeding of the International Symposium Interaction between water and living matter 6-10 October 1979. Publishing Hause “Nauka”, Moskow. II: 94-98.

Tsytsugina V.G. (1990). Chromosome mutagenesis in population of aquatic biota in the Black Sea, Aegean Sea and Danube and Dnieper 14 Rivers, 1986-1989 Proceeding of Seminar on comparative assessment of the environmental impact of radionuclides released during three major nuclear accidents: Kyshtym, Windscale, Chernobyl. Luxembourg, 1-5 October 1990. II: 895-904.

Tsytsugina V.G. (1992). Genetic processes in natural hydrobionts populations in regions of local anthropogenic pollution. Molismology of the Black Sea. Kiev. Publishing House “Naukova Dumka”. 154 -164 (in Russian).

Tsytsugina V.G. (1998). An indicator of radiation effects in natural populations of aquatic organisms. Radiat. Protection Dosimetry 75 (1 – 4): 171 – 173

Tsytsugina V.G., Polikarpov G.G. (1998). Pecularities of generative structure of hydrobionts populations and their radiosensitivity. Proceeding of Topical Meeting. Mol – Belgium, 01- 05 June. IUR. 3-11.

Tsytsugina V.G., Polikarpov G.G. (2005a). Rate of adaptation of hydrobionts populations with different reproductive strategies to anthropogenic pollution. Dopovidi Nat. Ak. Nauk Ukraine (1): 183-187. (in Russian).

Tsytsugina V.G., Polikarpov G.G. (2005b). An identification of “critical” species in the connection to ecological risk assessment. Dopovidi Nat. Ak. Naukk Ukraine (7): 196-200. (in Russian).

Tsytsugina V.G., Polikarpov G.G. (2005c). Study of the equivalence of radioactive and chemical contamination effects on natural populations of aquatic organisms: a methodology. Hydrobiological J. 41 (1): 78-89.

Tsytsugina V.G., Polikarpov G.G. (2006a). Assessment of expected reduction of crustacean populations adaptive possibilities at different levels of deleterious action. Marine Ecological J. 5 (1): 92-98 (in Russian).

Tsytsugina V.G., Polikarpov G.G. (2006b). The estimation of Chernobyl accident influence on the natural populations of the Black Sea basin hydrobionts by cytogenetic criteria. In: 20 years of the Chernobyl Accident: Past, Present and Future (eds. E.B. Burlakova and V.I. Naidich). New York. Nova Science Publishers Inc. Chapter 16: 287-302.

Volobuev V.G. (1978). B-chromosomes of mammals. Advantages of Modern Biology 86. (3) (6): 387-398 (in Russian).