Distribution of members of the family Enterobacteriaceae in the Istanbul Strait
Mine Çardak, Gülşen Altuğ
Canakkale Onsekiz Mart University, Faculty of Fisheries, Department of Marine Biology Canakkale, TURKEY
Istanbul University, Faculty of Fisheries, Department of Marine Biology Istanbul, TURKEY
The aim of this study was to determine the distribution of the Enterobacteriaceae members present in the sea water samples obtained from the stations selected at the Marmara Sea entry and the Black Sea exit of the Istanbul Strait and the effect of the physical parameters on the distribution of these indicator bacteria. A total of 126 unit isolates of the members of the Enterobacteriaceae were identified using API 20E (Biomereux) in the sea water samples obtained from the surface waters (0-30 cm) and various depths by monthly samplings from February 2006 to March 2007. Faecal and total coliform analyses were performed in order to state the bacterial pollution load using membrane filtration technique. Temperature, salinity, conductivity, pH and dissolved oxygen were measured by a CTD (SBE-15). The results of this study demonstrated that the highest bacterial abundance and pollution were present at the Marmara Sea entry of the Istanbul Strait, the number of bacteria determined below the depth of 20 m were higher than the deep discharge standards, and the deep discharge at the Marmara Sea entry of the Istanbul Strait was not attaining its purpose due to the lower layer waters were returning to the Marmara Sea instead of merging in the Black Sea, as pointed out by the bacterial levels.
Keywords: Enterobacterieaceae, Istanbul Strait, total coliform, faecal coliform
APHA (1999). Standard methods for the examination of water and waste water (20th ed.). Washington, DC: APHA.
Besiktepe, S.T., Sur, H.I., Ozsoy, E., Latif, M.A., Oguz, T. and Unluata, U. (1994). The circulation and hydrography of the Marmara Sea. Progress in Oceanography 34: 285-334.
Hitchins, A. D, Feng, P., Watkins, W. D, Ripley, S. R. and Chandler, L. A. (1992). Escherichia coli and the Coliforms. Bacteriological Analytical Manual, (7th ed.). Washington, DC: APHA.
Munro, P.M., Gauthier, M.J., Breittmayer, V.A. and Bongiovanni, J. (1989). Influence of some osmoregulaiton processes on starvation of Escherichia coli in seawater. Applied and Enviromental Microbiology 55: 2017-2014.
Tasdemir, Y. (2002). Marmara Denizi: Kirleticiler ve Çevre Açlsmdan Almabilecek Tedbirler, Uludaž Üniversitesi Miihendislik-Mimarlık Fakültesi Dergisi 7: 12-14.
Topcuoglu, S., Kut, D, Esen, N. , Ölmez (Ežilli), E., Küçükcezzar, R., Başsarı, A., Güngör, N., Kırbaçoglu, C. Marmara Denizi’nin sediment ve organizmalarda ağır metal kirliliği, Marmara Denizi 2000 Sempozyumu, 11-12 Kasım 2000, Ataköy Marina, Istanbul, TUDAV yayın no: 3 (Eds. B.Öztürk, M.Kad10élu, H.Öztürk) pp. 551-565.
Unluata, U. , Oguz, T. , Latif, M.A. and Ozsoy, E. (1990). On the physical oceanography of the Turkish Straits. In: the physical oceanography of sea straits, (Eds.): Pratt, L.J. Kluwer, Dortrecht, pp.25-60.