Investigations on raparin obtained from Rapana venosa (Valenciennes)

Lütfi Genç, Yıldız Özsoy, Erden Güler, Orhan N. Ulutin

University of Anadolu, Faculty of Pharmacy, Department of Pharmaceutical Technology, Eskişehir, TURKEY
University of Istanbul, Faculty of Pharmacy, Department of Pharmaceutical Technology, Istanbul, TURKEY
University of Istanbul, Cerrahpaşa Faculty of Medicine, Istanbul, TURKEY


Heparin was first isolated in 1916 from animal tissues. Raparin, heparin like substances, was isolated from Rapana venosa (Valenciennes) in 1991. In this study, raparin was extracted from hepatopankreas of Rapana venosa by three different techniques and fractionated through Sephadex G-50 Super fine column. Two fractions were obtained and their recalcification time, Heptest, Hepaclot methods and metachromatic activity were studied. All fractions showed activity in recalcification time assay and Raparin RKL10 FR I showed the greatest activity. All fractions were showed activity in Heptest but only raparin RKL10 FR II demonstrated considerable activity with Hepaclot test. Raparin RH500 Fr I showed metachromatic activity with azure A and toluidine blue dyes.

Keywords: Raparin, fractionation, biological activity assay, recalcification time, metachromatic activity


Burson, S.L., Fahrenbach, J., Frommhagen, L.H., Riccardi, B.A., Brown, R.A., Brockman, J.A., Lewry, H.V. and Stokstad, L.R. (1956) Isolation and purification of mactins, heparin like anticoagulants from mollusca, J. Am. Chem. Soc. 78: 5874-58781

Charles, A.F. and Scott, D.A. (1933) Studies on heparin I. the preparation of heparin, J. Biol. Chem. 102: 425-448.

Foster, R.H.K. and Nutley, N.J., (1992) The assay of heparin, J. Lab. Clin. Med., 27: 820-827

Güler, E. and Güven, K.C.(I989) Correlation between metachromatic and recalcification time methods for heparin assay, Fat Sci. Technol. 91:188-190.

Güven, K.C. and Ertan, G.(1984) The correlation equations for the assays of haperin in metachromatic and thrombin time methods I, Thrombosis Research 33: 197-201.

Güven, K.C., Özsoy, Y., Öztürk, B., Topaloğlu, B. and Ulutin, O.N. (1991) Raparin, e new heparinoid from rapana venosa (Valenciennes), Pharmazie 46: 547-548.

Howell, W.H. and Holt, F. (1918) Heparin as antiquagulant Am. J. Physiol. 47: 325 -329.

Jacques, L.B., Bruce-Milford, M. and Ricker, A.G. ( 1947) The metachromatic activity of heparin Rev. Can. Bioi. 6: 740 -754

Jaques, L.B ., Kavanagh, L. and Lavallee, A. (1967) A comparison of biological activities and chemical analyses for various heparin preparations, Arzneim. Forsch./Drug Res. 17: 774-778.

Kakkar, V.V. (1974) Low -dose heparin in the prevention of venous thromboembolism, Throm. Diath. Haem. 33: 87-96

Kuizenga, M.H. and Spaulding, L.B. (1943), The preparation of the highly active barium salt of heparin and its fractionation into two chemically and biologically different contituents. J. Biol. Chem. 148: 641-647.

Macintosh, F.C. (1941) A colorimetric method for the standardization of heparin preparations Biochem. J. 35: 776-782.

Mc Lean, J. (1916) The thromboblastic action of cephalin, Am. J. Physiol. 41: 250-256.

Ruan, C., Wu, Q., Wang, Z., Soria, J., Suria, C. and Caen, J.P. (1986) Heparin-like substances: acidic mucopolysaccharides from sea cucumbers and their biological effects, Med. J. Australia 144; HS 17-21.

Yin, E.T., Wessler, S. and Butler, J. (1973) Plasma heparin an unique, pactical submicrogram sensitive assay, J.Lab. Clin. Med. 81; 298-310.