A review on the problems of LAS determination methods in sea water

Kasım Cemal Güven, Burak Çoban

Turkish Marine Research Foundation (TUDAV), 34820, Beykoz, Istanbul, TURKEY
Department of Chemistry, Faculty of Science and Letters, Zonguldak Bulent Ecevit University, 67100, Zonguldak, TURKEY


In this paper, the research made on the factors which influence the determination of LAS in sea water by MBAS and HPLC methods was summarized. These factors are purity of LAB and LAS, degradation, light, storage time, salinity and methods used for analysis. These factors were discussed and a modification on the MBAS method was proposed.

Keywords: LAB, LAS, factors, purity, degradation, MBAS

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Abel, P.D. (1974) Toxicity of synthetic detergents on fish and aquatic invertebrates. J Fish Biol 6: 279-298.

Akıncı, S., Güven, K.C. (1997) A new determination method of anionic detergent in the sea water based on metachromasy and correlation between Azur A and methylene blue assays. Turkish J Mar Sci 3: 191-198.

Barr, T., Oliver, J., Stubbings, W.V. (1948) The determination of surface active agents in solution. J Soc Chem Ind 67: 45-48.

Bektaş, A., Güven, K.C. (2004) Determination of LAS by metachromatic method in seawater and reservoir water. Acta Pharm Turc 46: 83-94.

Black, J.D., Howes, D. (1980) Absorption, metabolism and excretion of anionic surfactants, in anionic surfactants (ed. C. Gloxhuber) Marcel Dekker Inc. New York.

Ceglarek, U., Efer, J., Schreiber, A., Zwanziger, E., Engewald, W. (1999) Determination of linear alkylbenzenesulfonates in communal wastewater by means of solid phase microextraction coupled with API-MS and HPLC-FLD. Fresenius J Anal Chem 365: 674-681.

Cosoviç, B., Voivodic, V., Plese, T. (1985) Electrochemical determination and characterization of surface active substance in freshwater. Water Res 19: 175-183.

Cripps, P.T., Eckert, J.M., Gibson, A. (1978) The determination of anionic detergents at p.p.b. levels by graphite furnace atomic absorption spectrometry. Anal Chim Acta 87: 97-101.

Çetintürk, K., Güven, K. C. (2009) The effect of sodium chloride concentration on the assay of LAS in seawater by MBAS methods. J Black Sea/ Mediterranean Environment 15(2): 33-45.

Degens, P.N., Evans, H.C., Kommer, J.D., Winsor, P.A. (1953) Determination of sulfate and sulfonate an ion-active detergents in sewage. J Appl Chem (London) 3: 54-61.

Ehrlich, P. (1887) Beitrage zur Kenntnis der Anilinfarbungen und ihrer verwendung in der mikroskopischen technik. Archiv F Mikrosk Anotomie 13: 263-277.

Epton, S.R. (1948) New method for the rapid titrimetric analysis of sodium alkyl sulfates and related compounds. Trans Faraday Soc 44: 226-230.

Evans, H.C. (1950) Determination of anionic synthetic detergents in sewage. J. Soc. Chem. Ind. (London) 69(Suppl. 2): 76-80.

Gonzalez-Mazo, E., Gomez-Parra, A. (1996) Monitoring anionic surfactants (LAS) and their intermediate degradation products in the marine environment. Trends Anal Chem 15: 375-380.

Güven, K.C., Cumalı, S. (2007) The influence of salinity of seawater on the determination of LAS by MBAS methods. Acta Pharm Sci 49: 161-166.

Güven, K.C., Coban, B. (2013) LAS pollution of the Sea of Marmara, Golden Horn and Istanbul Strait (Bosphorus) during 2004-2007. J Black Sea/Mediterranean Environment 19: 331˗354.

Güven, K.C., Çubukçu, N., İpteş, M., Özyalvaç, M., Cumalı, S., Nesimigil, F., Yalçın, A. (2008) Oil and detergent pollution in surface waters and sediments of the Istanbul Strait, Golden Horn, Izmit Bay (Sea of Marmara), Çanakkale Strait, Ali Ağa (Aegean Sea) in 2005-2007. J Black Sea/Mediterranean Environment 14: 205-220.

Güven, K.C., Gürakın, N., Akıncı, S., Bektaş, A., Öz, V. (1994) Metachromatic method for the assay of LAS in town and sea water. Acta Pharm Turc 36: 136- 140.

Güven, K.C., Nesimigil, F., Cumali, S., Yalçın, A., Gazioğlu, C., Coban, B. (2010) Anionic detergent LAS pollution and discharged amount from Turkish coasts to the Black Sea during 2004-2007. J Black Sea/Mediterranean Environment 16: 5-24.

Han, S., Yang, H. (1992) Degradation of LAS from the detergent was water by the immobilized mixed bacteria culture. Weishengwuxue Tongbao. 19: 88-91 C.A. 117: 239037 (1993).

Hashim, M.A., Kulandai, J., Hassan, R.S. (1992) Biodegradability of branched alkylbenzene sulphonates. J Chem Tech Biotechnol 54: 207-214.

Hellman, H. (1979) Analytische Bestimmung von Aniontensiden in Schwebund Sinktoffen sowie Klarschlammen. Analytische Chemie 295: 393-397.

Hon-Nami, H., Hanya, T. (1978) Gas liquid chromatographic mass spectrometric determination of alkylbenzenesulphanates in river water. J Chromatography 161: 202-205.

Hon-Nami, H., Hanya, T. (1980) Linear alkylbenzene sulfonates in river, estuary and bay water. Water Research 14 (9): 1251-1256.

Ivanov, V.N., Prashin, Y.O. (1985) Determination of anionic and nonionic surfactants using ion-selective electrodes. Zavod Lab 51: 6-8.

Koç, H., Güven, K.C. (2002) Toxicity and identification of LAS in tissue of rainbow trout (Oncorhynchus mykiss). Turkish J Mar Sci 8: 197-208.

Koç, H., Güven, K.C., Gezgin, T. (2001) Determination of LAS by HPLC method. Turkish J Mar Sci 7: 85-95.

Koç, H., Güven, K.C., Gezgin, T. (2002) Degradation of LAS in distilled, tap and sea water. Turkish J Mar Sci 8: 91-102.

Lison, L. (1935) Etude sur metachromasie colorant metachromatiques et substances chromotropes. Archive de Biologie 46: 599-668.

Marcomini, A., Giger, W. (1987) Simultaneous determination of linear alkylbenzene sulphonates, alkylphenol polyethoxylates and nonyl phenol by high performance liquid chromatography. Anal Chem 59: 1709-1715.

Okpokwasili G.O., Olisa A.O. (1991). River water biodegradability of surfactants in liquid detergent and shampoos. Water Res 25: 1425-1429.

Quiroga J.M., Sales, D. (1990) Experimental variables in biodegradation of surfactant in marine environment. Bull Environ Contam Toxicol 44(6):851-8.

Raymundo, C.C., Preston, M.R. (1992) The distribution of linear alkylbenzenes in coastal and estuarine sediments of the western North sea. Mar Poll Bull 24: 138-146.

Rubio, J.A., Gonzalez-Mazo, E., Gomez-Parra, A. (1996) Sorption of linear alkylbenzenesulfonates (LAS) on marine sediment. Mar Chem 54: 171-177.

Sablayrolles, C., Montrejaud-Vignoles, M., Silvestre, J., Treilhou, M. (2009) Trace determination of linear alkylbenzene sulfonate Application in artificially polluted soil-carrot system. Int. J. Anal. Chem. 2009: Article ID 404836, 6 pages.

Spehar, R.L., Holcombe, G.W., Carlson, R.W., Drummod, R.A., Yount, J.D., Pickering, Q.H. (1979) Effect of pollution on fresh water fish. J Water Pollution Control Federation 51: 1616-1684.

Srivastava, V.K., Misra, B.B. and Tripathi, A.M. (1977) Effect of acid and inorganic salts on metachromasia induced by hetropolyanions. Transition Met Chem 2: i06- i08.

Standard Methods for examination of water and wastewater (1992) American Public Health Assoc. New York.

Swisher, R., 1987. Surfactant Biodegradation, 2nd ed. Marcel Dekker Inc, New York.

Terzic, S., Hrsak, D., Abel, M. (1992) Enrichment and isolation of linear alkylbenzenesulfonate (LAS) degrading bacteria from estuarine and coastal waters. Mar Poll Bull 24: 199-204.

Topcuoglu, S., Birol, E. (1982) Bioaccumulation of sodium alkyl sulfate zinc chloride and their mixture in young goby Proterorhinus marmoratus Pall. Turkish J Nuclear Sci 9: 100-107.

Unlu, Y., Topçuoğlu, S. (1977) Determination of LC50 and estimation of safe level of LAS detergents for larvae of two fish species in Küçük Çekmece Lagoon. CNAM Report No: 171.

Villar, M., Callejon, M., Jimenez, J.E., Alonso, E., Guiram, A. (2009) New rapid methods for determination of total LAS in sewage sludge by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Ann Chim Acta 634: 267-271.

Wood, P.C., Ayres, P.A. (1977) Artificial Sea Water for Shell Fish Tanks. Ministry of Agriculture Fisheries and Food Directorate of Fisheries Research ISSN 0772-6699.