TOXICITY AND IDENTIFICATION OF LAS IN TISSUE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Toxicity and identification of LAS in tissue of rainbow trout (Oncorhynchus mykiss)

Hüseyin Koç, Kasim Cemal Güven

Istanbul University, Institute of Marine Sciences and Management. Vefa, 34470, Istanbul, TURKEY

Abstract

Toxicity and identification of LAS were investigated on rainbow trout (Oncorhynchus mykiss) exposed to LAS at various concentration levels in aquarium. The lethal level of LAS was 12.5 mg/L in single dose and 13.5 mg/L in progressive doses. The accumulation of LAS in the organs such as gill, heart, stomach and liver were determined by metachromatic method with azur A and toluidine blue. The LAS absoIbed on tissue is colored red in examination on microscope. This is the first record in the literature using metachromasy for biological trace of LAS in tissue.

Keywords: Toxicity, LAS, metachromasy, rainbow trout