Macroinfaunal invertebrates associated to Cladocora caespitosa (Cnidaria: Anthozoa) in Gökçeada (northern Aegean Sea)

Macroinfaunal invertebrates associated to Cladocora caespitosa (Cnidaria: Anthozoa) in Gökçeada (northern Aegean Sea)

Tancrède Barraud, Bayram Öztürk

ORCID IDs: T.B. 0000-0001-6621-0922; B.Ö. 0000-0001-7844-2448

Marine Ecology Department, University of the French West Indies and Guiana, Pointe-à-Pitre 97157, Guadeloupe, FRANCE
Marine Biology Programme, Institute of Sciences and Technology, Istanbul University, 34134, Istanbul, TÜRKİYE
Marine Biology Department, Faculty of Aquatic Sciences, Istanbul University, 34134 Istanbul, TÜRKİYE
Turkish Marine Research Foundation, 34820 Istanbul, TÜRKİYE


Macro-invertebrates associated with stony coral Cladocora caespitosa were studied around Gökçeada Island (northern Aegean Sea) up to a depth of 15.5 m during the spring of 2019. Twelve dead/partially dead colonies were collected, to which 68 infaunal taxa were found to be associated. The overall number of associated taxa were updated to 397 (406 with macroalgae included). The present study added 26 taxa to the total number of associates. The most abundant group was Polychaeta (61 %), followed by Mollusca (16 %) and Crustacea (9 %). The most abundant species in the area were Syllis gracilis, Perinereis cultrifera, Vermiliopsis striaticeps and Serpula vermicularis. Similar patterns of aggregation were recognized between polychaetes and biota data via PCO plots. DISTLM procedure indicated that 94 % of biota variation was explained by the number of recently necrotized polyps and living polyps. The impact of temperature on biota was underlined in the present study (ANOSIM), however the extent of influence by sea surface temperature on the associated biota remains unclear.

Keywords: Cladocora caespitosa, stony coral, macro-infauna, polychaetes, multivariate analysis

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Sexual reproduction of Anemonia sulcata (Pennant, 1777) in Moroccan western Mediterranean coast

Sexual reproduction of Anemonia sulcata (Pennant, 1777) in Moroccan western Mediterranean coast

Mouna Daoudi, Rachid El Halimi, Benlahcen Rijal Leblad, Oussima El Haroufi

ORCID IDs: M.D. 0000-0003-2032-7345; R.E.H. 0000-0002-3846-8827; B.R.L. 0000-0002-9713-9492; O.E.H. 0000-0002-5785-9583

Laboratory of Fisheries, National Institute of Fisheries Research, Tangier, MOROCCO
Department of Mathematics and Statistics, Faculty of Science and Technic of Tangier, University Abdelmalek Essaadi (UAE), MOROCCO
Laboratory of Monitoring of Coastal Environment, National Institute of Fisheries Research, Tangier, MOROCCO
Department of Earth Sciences, Faculty of Sciences and Technic of Tangier, University Abdelmalek Essaadi (UAE), MOROCCO


The sexual reproduction of the sea anemone, Anemonia sulcata (Pennant, 1777), was studied for the first time at Cape Mazari in the Moroccan Mediterranean coast over an annual cycle from May 2017 to May 2018. A total of 330 individuals of A. sulcata were sampled during 11 months. The sampled individuals were measured, weighed, and fixed for histological analyses. The pedal disc diameter (PDD) of the species ranged from 1.2 cm to 6.6 cm with a mean value of 2.7 ± 0.6 cm. The weight of the species varied from 2.6 g to 94.0 g with a mean value of 19.9 ± 12.7 g. Results of histological analyses indicated a sex ratio of 2.3 female: 1 male. Gonads were observed generally throughout the study period and mature ones were encountered from February to June. Five possible maturity stages of A. sulcata for each gender were recorded. Based on this study, the exploitation of A. sulcata can be managed in the Moroccan Mediterranean coast by the application of the biological rest period from February to June as well as monitoring its weight.

Keywords: Anemonia sulcata, sexual reproduction, biological rest period

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Assessment of the genotoxic effect of thiamethoxam in Cyprinus carpio by the micronucleus and Comet assays

Assessment of the genotoxic effect of thiamethoxam in Cyprinus carpio by the micronucleus and Comet assays

Ayşegül Ergenler, Funda Turan

ORCID IDs: A.E. 0000-0001-9186-3909; F.T. 0000-0002-0257-6009

Faculty of Marine Science and Technology, Iskenderun Technical University, 31200, Iskenderun, Hatay, TÜRKİYE


Pesticides are compounds formed by natural or various chemical processes that kill harmful organisms, remove pests from their environment, reduce population density or completely eliminate them. Monitoring for toxic effects and screening for different insecticides is vital and crucial for reducing adverse effects on aquatic organisms and public health. Therefore, in this study, we aimed to determine genotoxic effect of thiamethoxamine in a model fish species, Cyprinus carpio, using the micronucleus test and Comet assay. Common carp (average weight of 1.56±0.31g) were exposed to control and three different concentrations of thiamethoxamine (1.0, 1.5 and 2.0 mg L-1) based on previously detected aquatic environmental concentrations for ten days. At the end of study, the Damage frequency (%), Arbitrary unit and Genetic damage index (%) were evaluated in gill and liver cells of carp by Comet assay. Micronucleus frequencies and erythrocyte abnormalities were also determined in erythrocytes cells of carp by micronucleus test. The highest micronucleus frequency and erythrocyte abnormalities were significantly observed in 2.0 mg L-1 group (p<0.001) and, the highest damage frequencies (%) as 74.000±1.732 and 68.000±1.732 were significantly observed in 2.0 mg L-1 group in gill and liver cells, respectively (p<0.001). Our results revealed significant increases in the frequencies of micronuclei and DNA strand breaks in C. carpio, following exposure to thiamethoxamine, thus demonstrated the genotoxic potential of this pesticide on fish.

Keywords: DNA damage, thiamethoxam, micronucleus test, Comet assay, pesticide

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COMMENTS ON: Okudan, E.Ş., Dural, B., Erdugan, H., Aktan, Y., Demir, V., Aysel, V. (2021) New record of the benthic marine mucilage aggregates Phaeocystis giraudii from Turkish Eastern Mediterranean Sea. Journal of the Black Sea/Mediterranean Environment 27(2): 124-139

COMMENTS ON: Okudan, E.Ş., Dural, B., Erdugan, H., Aktan, Y., Demir, V., Aysel, V. (2021) New record of the benthic marine mucilage aggregates Phaeocystis giraudii from Turkish Eastern Mediterranean Sea. Journal of the Black Sea/Mediterranean Environment 27(2): 124-139

Neslihan Balkis-Ozdelice, Turgay Durmus, Muharrem Balci

ORCID IDs: N.B.O. 0000-0001-8030-7480; T.D. 0000-0002-8242-1823; M.B. 0000-0001-9373-6647

Department of Biology, Faculty of Science, Istanbul University, Vezneciler, Istanbul, TÜRKİYE

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Balkis-Ozdelice, N., Durmus, T., Balci, M. (2021) A preliminary study on the intense pelagic and benthic mucilage phenomenon observed in the Sea of Marmara. International Journal of Environment and Geoinformatics 8(4): 414-422.

Demir, V. (2011) Marine Biological Diversity Assessment for Marine Conservation Planning in Antalya-Kas Using Decision. Ph.D. Thesis, Institute of Marine Sciences and Management, Istanbul University, Turkey (in Turkish).

Okudan, E.Ş., Dural, B., Erdugan, H., Aktan, Y., Demir, V., Aysel, V. (2021) New record of the benthic marine mucilage aggregates Phaeocystis giraudii from Turkish Eastern Mediterranean Sea. Journal of Black Sea/Mediterranean Environment 27(2): 124-139.

Macrobenthic fauna of soft-bottom in the eastern sector of the Gulf of Oran (western Algeria, Mediterranean Sea)

Macrobenthic fauna of soft-bottom in the eastern sector of the Gulf of Oran (western Algeria, Mediterranean Sea)

Ahmed Kerfouf, Habib Meliani, Patricio R. de los Ríos-Escalante, Kamel Belhadj Tahar, Denis Françoise

ORCID IDs: A.K. 0000-0002-4466-1201; H.M. 0000-0002-7051-6059; P.D.L.R. 0000-0001-5056-7003; K.B.T. 0000-0002-0711-9296; F.D. 0000-0002-0006-8238

Laboratory of Eco-development of Space, University of Djillali Liabes, Sidi Bel Abbès, 22000, ALGERIA
Departamento de Ciencias Biológicas y Químicas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Casilla 15-D, Temuco, CHILE
Núcleo de Estudios Ambientales UC Temuco, CHILE
Le Mans University- Laboratory BOREA MNHN, CNRS 8067, SU, IRD 207, UCN, UA- Concarneau, FRANCE


Research of benthic macrofauna of the Algerian west coasts is generally rare and mainly concerns a single species. The present work created an inventory of the macrobenthos explaining their distribution in the eastern sector of the Gulf of Oran. A total of 506 individuals sampled at 14 sites in winter 2019 were identified as four main taxa (279 polychaetes, 154 crustaceans, 41 echinoderms, and 32 molluscs). The taxonomic analysis made it possible to inventory 112 species (25 polychaetes, 61 crustaceans, 18 molluscs and 8 echinoderms). Polychaetes were dominant in all stations. Four bio-sedimentary facies (sand, sanded gravel, graveled sand and gravel) were identified by Ascending Hierarchical Classification, diversity indices, and sedimentary analysis and the zoological and ecological characteristics of the main soft-bottom communities of the study area were described.

Keywords: Macrofauna, macrobenthos, softbottom, sedimentary facies, Gulf of Oran, western Algeria

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Bioaccumulation of heavy metals in Poecilia reticulata (guppy fish): an important biotic component of food chain

Bioaccumulation of heavy metals in Poecilia reticulata (guppy fish): an important biotic component of food chain

Muhammad Uzair Khan, Moinuddin Ahmed, Kanwal Nazim, Saquib Ejaz Hussain

ORCID IDs: M.U.K. 0000-0003-1765-0803; M.A. 0000-0002-9502-5328; K.N. 0000-0002-3684-0779; S.E.H. 0000-0003-2022-3845

Marine Reference Collection and Resource Centre, University of Karachi, PAKISTAN
Department of Earth and Environmental Systems, Indiana State University, IN, USA
Society for Environment & Mangrove Protection Welfare Association, PAKISTAN
E.H.S. Services, Karachi, PAKISTAN


Fish are the important part of aquatic food chain and also the essential part of protein in human beings. They have capability to accumulate pollutants from ambient water which directly or indirectly poses the adverse effect on humans. The present study reveals the significant effects of untreated industrial wastes on fish species Poecilia reticulata. For this purpose an aquarium-based trial was conducted with five different types of industrial wastes including chemical industry sludge, chemical industry ash, boulder slag, converter slag and marble waste powder for five heavy metals, i.e. Zn, Fe, Co, Mn and Pb. The water and tissue samples were analyzed to check the possible accumulation of metals in the food chain. Converter slag showed overall better results in terms of growth among treatments while chemical industrial sludge-ash showed deleterious effects on overall growth parameters of the species as number of fry > number of adult > dry weight > fresh weight. The number of adults and fries of P. reticulata were significantly reduced (p<0.001) while, fresh and dry weights were also reduced significantly (p<0.05, fresh wt and p<0.01, dry wt) in ash treatment. Fish showed greater capacity to accumulate metals when compared to ambient water with the highest accumulation for Fe and the lowest for Pb. Studies on fish for heavy metal accumulation are important with respect to the human consumption through the food chain.

Keywords: Industrial wastes, heavy metals, water pollution, Poecilia reticulata, food chain

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First documented records of Apolemia cf. uvaria (Lesueur, 1815) (Cnidaria: Siphonophorae) and Cestum veneris Lesueur, 1813 (Ctenophora: Cestidae) in the Lebanese waters, the eastern Mediterranean Sea

First documented records of Apolemia cf. uvaria (Lesueur, 1815) (Cnidaria: Siphonophorae) and Cestum veneris Lesueur, 1813 (Ctenophora: Cestidae) in the Lebanese waters, the eastern Mediterranean Sea

Ali Badreddine, Ghazi Bitar, Stefano Piraino

ORCID IDs: A.B. 0000-0003-4576-7400; G.B. 0000-0003-0270-5613; S.P. 0000-0002-8752-9390

Tyre Coast Nature Reserve, Department of Biology, Tyre-South, LEBANON
Faculty of Sciences, Lebanese University, Hadath, Beirut, LEBANON
Università del Salento, DISTEBA, 73100 Lecce, ITALY


Two jellyfish species are documented for the first time from the Lebanese waters. A string jellyfish, siphonophore, Apolemia cf. uvaria, was observed first on 25 May 1993, at 5 m depth, off Tripoli (north of Lebanon) and recently on 25 April 2021 photographed off Saida (south Lebanon) at 16 m depth. The other species, a rare ctenophore, venus girdle, Cestum veneris, was observed and photographed at around 2 m depth off Tyre (south Lebanon), on 4 March 2021.

Keywords: Siphonophorae, Ctenophora, Apolemia cf. uvaria, Cestum veneris, Lebanese waters, eastern Mediterranean Sea

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First record of the non-indigenous sohal surgeonfish Acanthurus sohal (Forsskål, 1775) (Chordata: Acanthuridae) from the Lebanese waters, eastern Mediterranean Sea

First record of the non-indigenous sohal surgeonfish Acanthurus sohal (Forsskål, 1775) (Chordata: Acanthuridae) from the Lebanese waters, eastern Mediterranean Sea

Ali Badreddine, Ghazi Bitar, Ricardo Aguilar

ORCID IDs: A.B. 0000-0003-4576-7400; G.B. 0000-0003-0270-5613; R.A. 0000-0001-6615-8626

Tyre Coast Nature Reserve, Department of Biology, Tyre-South LEBANON
Faculty of Sciences, Lebanese University, Hadath, Beirut, LEBANON
OCEANA, Gran Via 59, 9, 28013, Madrid, SPAIN


The non-indigenous sohal surgeonfish Acanthurus sohal is reported for the first time from the Lebanese waters. On 29 April 2021, a fisherman captured one individual of the species at 5 m depth in Tyre, south Lebanon. This record constitutes the first one in the Lebanese waters and the third in the Mediterranean Sea.

Keywords: Acanthurus sohal, non-indigenous fishes, Lebanese waters, eastern Mediterranean Sea

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First record of a praniza larva of the family Gnathiidae (Crustacea, Isopoda) living on Chromogobius zebratus (Pisces, Gobiidae) in the eastern Mediterranean Sea

First record of a praniza larva of the family Gnathiidae (Crustacea, Isopoda) living on Chromogobius zebratus (Pisces, Gobiidae) in the eastern Mediterranean Sea

Furkan Durucan

ORCID ID: 0000-0002-6168-2135

German Centre for Marine Biodiversity Research (DZMB), Senckenberg am Meer, Wilhelmshaven, GERMANY
Department of Aquaculture, Isparta University of Applied Sciences, Isparta, 32260, TÜRKİYE


In this study, a praniza larva of a gnathiid isopod was reported for the first time from the eastern Mediterranean Sea of Turkey, Antalya. In particular, it was found on caudal peduncle of the fish Chromogobius zebratus (Kolombatovic, 1891).

Keywords: Ectoparasite, Gnathiidae, Turkey, Levantine Sea

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Vol.28 – 2022 – No.1

Introductory pages

Sexual reproduction of Anemonia sulcata (Pennant, 1777) in Moroccan western Mediterranean coast
Mouna Daoudi, Rachid El Halimi, Benlahcen Rijal Leblad, Oussima El Haroufi

Macroinfaunal invertebrates associated to Cladocora caespitosa (Cnidaria: Anthozoa) in Gökçeada (northern Aegean Sea)
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Assessment of the genotoxic effect of thiamethoxam in Cyprinus carpio by the micronucleus and Comet assays
Ayşegül Ergenler,  Funda Turan

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Bioaccumulation of heavy metals in Poecilia reticulata (guppy fish): an important biotic component of food chain
Muhammad Uzair Khan, Moinuddin Ahmed, Kanwal Nazim, Saquib Ejaz Hussain

First documented records of Apolemia cf. uvaria (Lesueur, 1815) (Cnidaria: Siphonophorae) and Cestum veneris Lesueur, 1813 (Ctenophora: Cestidae) in the Lebanese waters, the eastern Mediterranean Sea
Ali Badreddine, Ghazi Bitar, Stefano Piraino

First record of the non-indigenous sohal surgeonfish Acanthurus sohal (Forsskål, 1775) (Chordata: Acanthuridae) from the Lebanese waters, eastern Mediterranean Sea
Ali Badreddine, Ghazi Bitar, Ricardo Aguilar

First record of a praniza larva of the family Gnathiidae (Crustacea, Isopoda) living on Chromogobius zebratus (Pisces, Gobiidae) in the eastern Mediterranean Sea
Furkan Durucan

COMMENTS ON: Okudan, E.Ş., Dural, B., Erdugan, H., Aktan, Y., Demir, V., Aysel, V. (2021) New record of the benthic marine mucilage aggregates Phaeocystis giraudii from Turkish Eastern Mediterranean Sea. Journal of the Black Sea/Mediterranean Environment 27(2): 124-139
Neslihan Balkis-Ozdelice, Turgay Durmus, Muharrem Balci